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KMID : 0364219920350040466
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Korean Journal of Zoology
1992 Volume.35 No. 4 p.466 ~ p.473
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Purification and Characterization of Storage Protein-1 from Galleria mellonella
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Lee Yong-Ho
Yoe Sung-Moon
Kim Hak-Ryul
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Abstract
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Storage protein-1 (SP-1) of Gallerio mellonella was identified in hemolvmph and fat body by electrophoresis. SP-1 was purified from hemolvmph by KBr density gradient ultracentrifugation , DEAE-cellulose (DE52) ion-exchange chromatography, and gel permeation chromatography (Sephadex G-200). Purity of SP-1 was confirmed by Non-SDS PAGE and electron microscope. SP-1 is 9.4 nm in diameter and regular octahedron in shape. SP-1 has isoelectric point of 5.7 and native molecular weight of 365 K dalton and is composed of one type of subunit with molecular weight of 82 K dalton. Ttiacylslvcerol and phospholipid were found to be maior lipid components in SP-1.
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KEYWORD
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Purification, Gemolymph, Storage protein-1 Galleria mellonella
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